Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: Photographs (magnification 400X) depicting macrophage (brown stain; arrows) in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO. T = tubule; G = glomerulus.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Staining

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: Renal cortical active IL-18 protein levels in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques:

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: Quantitative IL-18 receptor (IL-18R) mRNA expression represented as a percentage of β-actin in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: Gel photograph and densitometric analysis of active caspase-1 expression represented as a percentage of GAPDH in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Expressing

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: A. Photographs (magnification 400X) depicting renal cortical IL-18 production (red) and distal tubular staining (Peanut Agglutinin; green) in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO. White arrows indicate IL-18 staining overlying tubules. T = Tubules.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Staining

Journal: PLoS ONE

Article Title: Renal IL-18 Production Is Macrophage Independent During Obstructive Injury

doi: 10.1371/journal.pone.0047417

Figure Lengend Snippet: A. Photographs (magnification 400X) depicting renal cortical IL-18R production (red) and distal tubular staining (Peanut Agglutinin; green) in vehicle treated and macrophage depleted mice exposed to sham operation or one week of UUO. White arrows indicate IL-18R staining overlying tubules. T = Tubules.

Article Snippet: The tissues were then blocked with 1% bovine serum albumin (BSA) and incubated with a a rat anti-mouse macrophage antibody (F4/80; 1∶100, Angio-Proteomie, Boston, MA) for 90 min at room temperature.

Techniques: Staining